promix cmv peptide pool px-cmv  (Proimmune)

Journal: Nature Communications

Article Title: Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function

doi: 10.1038/s41467-018-05816-y

Figure Lengend Snippet: HSPC-pDCs have the capacity to adopt an antigen-presenting phenotype. a Expression levels of the indicated maturation APC markers on IFN-primed/unprimed HSPC-pDCs at day 21 + 3 after stimulation with the TLR7 agonist R837. b Graphical illustration of the experimental setup for T cell stimulatory capacity of HSPC-pDCs. HSPC-pDCs (IFN-primed and TLR7-activated) were pulsed with a mix of peptides derived from CMV (CMV ProMix) or left untreated (UT). After 3 h, cells were washed and co-cultured with allogeneic PBMCs harvested from CMV-seropositive donors and T cell activity was subsequently evaluated by measuring levels of IFN-γ in the supernatant 24 h later. c Levels of IFN-γ produced by PBMCs from three CMV-seropositive donors after co-culture with pDCs loaded with CMV ProMix. d Levels of IFN-γ produced by the same three PBMC donors mixed in a 10:1 ratio with three HSPC-pDC donors (IFN-primed and TLR7-activated) for 3 h with or without a pulse of CMV ProMix. e Blood pDCs or HSPC-pDCs were cultivated for 1, 3, 5, 8, and 12 days in medium supplemented with IL-3. At each time point, the viability was assessed by flow cytometry. f Comparison of area under curve of HSPC-pDCs and blood pDCs in the different conditions tested. Data shown are ±SEM of three ( a – d ) or four ( e – f ) donors. Statistical analysis was performed using regular two-way ANOVA ( e , f ) followed by Bonferroni post hoc test

Article Snippet: After 24 h stimulation, HSPC-pDCs were pulsed with 1 μg per peptide/mL of CMV (ProMix TM CMV Peptide Pool, ProImmune) or left untreated.

Techniques: Expressing, Derivative Assay, Cell Culture, Activity Assay, Produced, Co-Culture Assay, Flow Cytometry, Comparison

Journal: Cell host & microbe

Article Title: Defective HIV-1 proviruses are expressed and can be recognized by cytotoxic T lymphocytes which shapes the proviral landscape

doi: 10.1016/j.chom.2017.03.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ProMix CMV Peptide Pool , ProImmune , PX-CMV.

Techniques: Functional Assay, Purification, Virus, Recombinant, Cell Isolation, Isolation, cDNA Synthesis, Amplification, Cloning, Enzyme-linked Immunosorbent Assay, Software

Journal: Cytokine

Article Title: Maintenance of CMV-specific CD8 + T cell responses and the relationship of IL-27 to IFN-? levels with aging

doi: 10.1016/j.cyto.2012.11.024

Figure Lengend Snippet: PBMCs were purified from the peripheral blood of young (age ≤ 40) and elderly (age ≥ 65) donors infected with CMV and stimulated for 6 hours with or without a CMV pp65 peptide pool in the presence of Golgi plug during the last 5 hours of stimulation. Stimulated cells were fixed and permeabilized using appropriate buffers followed by staining with antibodies to CD3, CD8, IFN-γ and TNF-α (BD Pharmingen). Stained cells were analyzed on an LSRII® flow cytometer. (A) Representative data showing CD8+ T cells producing IFN-γ and/or TNF-α in response to CMV pp65 peptides. (B–E) The frequency of CD8+ T cells producing IFN-γ and/or TNF-α (B), IFN-γ only (C), TNF-α only (D) and both IFN-γ and TNF-α (E) in response to CMV pp65 peptides in young (n = 12) and elderly people (n = 14). Bars and error bars indicate mean and standard error of mean (SEM), respectively.

Article Snippet: To measure cytokine production by CMV-specific CD8 + T cells, PBMCs from CMV-positive individuals were stimulated for 6 hours with or without a CMV pp65 peptide pool (ProMix TM HCMVA (PP65), Proimmune, Sarasota, FL) in the presence of Golgi plug (BD Pharmingen, San Diego, CA) during the last 5 hours of stimulation.

Techniques: Purification, Infection, Staining, Flow Cytometry

Journal: Cytokine

Article Title: Maintenance of CMV-specific CD8 + T cell responses and the relationship of IL-27 to IFN-? levels with aging

doi: 10.1016/j.cyto.2012.11.024

Figure Lengend Snippet: PBMCs were purified from the peripheral blood of young (age ≤ 40) and elderly (age ≥ 65) donors infected with CMV and stained with CFSE. Stained cells were stimulated for 7 days with or without a CMV pp65 peptide pool. Cell proliferation was analyzed on an LSRII® flow cytometer. (A) Representative data showing CD8+ T cell proliferation in response to CMV pp65 peptides. (B) The frequency of CD8+ T cells that proliferated in response to CMV pp65 peptides in young (n = 11) and elderly (n =15) people. Bars and error bars indicate mean and SEM, respectively. (C–D) Correlation of the frequencies of proliferating and cytokine producing CD8+ T cells in young and elderly people. P values were obtained using the unpaired t-test (B) and the Pearson correlation analysis (C–D).

Article Snippet: To measure cytokine production by CMV-specific CD8 + T cells, PBMCs from CMV-positive individuals were stimulated for 6 hours with or without a CMV pp65 peptide pool (ProMix TM HCMVA (PP65), Proimmune, Sarasota, FL) in the presence of Golgi plug (BD Pharmingen, San Diego, CA) during the last 5 hours of stimulation.

Techniques: Purification, Infection, Staining, Flow Cytometry